Imaging of HIV entry and egress

Anupriya Aggarwal; Stuart G Turville

doi:10.1071/MA14035

RESEARCH ARTICLE

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Imaging of HIV entry and egress

Anupriya Aggarwal ^A and Stuart G Turville ^A ^B

+ Author Affiliations

- Author Affiliations

^A Laboratory of HIV Biology
Immunovirology and Pathogenesis Program
The Kirby Institute
University of New South Wales
NSW 2010, Australia

^B Laboratory of HIV Biology
Immunovirology and Pathogenesis Program
The Kirby Institute
University of New South Wales
NSW 2010, Australia
Tel: +61 2 8382 4950
Fax: + 61 2 8382 4945
Email: sturville@kirby.unsw.edu.au

Microbiology Australia 35(2) 107-109 https://doi.org/10.1071/MA14035
Published: 15 May 2014

Abstract

During the early stages of HIV research, imaging of HIV was confined to the ultrastructural level¹. These early images gave us glimpses of the viral life cycle from the early stages of entry, with HIV detection at the plasma membrane and within endocytic/vesicular compartments through to different sites of viral assembly/budding in CD4 T cells and macrophages¹^–⁵. Whilst these previous snapshots of fixed specimens were seminal in nature, the increasing use of fluorescent proteins (FP) and key advances in fluorescent microscope technologies now give us the tools to test hypotheses in not only live cells, but also with live virus that could also be tracked in real-time. Herein we review the advances in HIV tracking, with an emphasis on recent observations that link HIV to the cortical F-actin network during HIV egress.

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