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RESEARCH ARTICLE

Activated sludge foaming: can phage therapy provide a control strategy?

Steve Petrovski A B and Robert Seviour A C
+ Author Affiliations
- Author Affiliations

A Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, Vic. 3086, Australia

B Email: steve.petrovski@latrobe.edu.au

C Email: r.seviour@latrobe.edu.au

Microbiology Australia 39(3) 162-164 https://doi.org/10.1071/MA18048
Published: 7 August 2018

Abstract

Foaming in activated sludge systems is a global problem leading to environmental, cosmetic and operational problems. Proliferation of filamentous hydrophobic bacteria (including the Mycolata) are responsible for the stabilisation of foams. Currently no reliable methods exist to control these. Reducing the levels of the filamentous bacteria with bacteriophages below the threshold supporting foaming is an attractive approach to control their impact. We have isolated 88 bacteriophages that target members of the foaming Mycolata. These double stranded DNA phages have been characterised and are currently being assessed for their performance as antifoam agents.


References

[1]  Seviour, R. and Nielsen, P.H. (2010) Microbial ecology of activated sludge. IWA publishing.

[2]  Jenkins, D. et al. (2003) Manual on the causes and control of activated sludge bulking, foaming, and other solids separation problems. CRC Press.

[3]  Petrovski, S. et al. (2011) An examination of the mechanism for stable foam formation in activated sludge systems. Water Res. 45, 2146–2154.

[4]  Soddell, J. (1998) Foaming. In The microbiology of activated sludge. pp. 161–202, Kluwer Academic Publishers, Dordrecht.

[5]  de los Reyes III (2010) Foaming. In The Microbial Ecology of Activated Sludge. pp. 215–258. IWA Publishing, London.

[6]  Seviour, R.J. et al. (2008) Ecophysiology of the Actinobacteria in activated sludge systems. Antonie van Leeuwenhoek 94, 21–33.
Ecophysiology of the Actinobacteria in activated sludge systems.Crossref | GoogleScholarGoogle Scholar |

[7]  Nielsen, P.H. et al. (2009) Identity and physiology of filamentous bacteria in activated sludge. FEMS Microbiol. Rev. 33, 969–998.

[8]  Kragelund, C. et al. (2007) Ecophysiology of mycolic acid-containing Actinobacteria (Mycolata) in activated sludge foams. FEMS Microbiol. Ecol. 61, 174–184.
Ecophysiology of mycolic acid-containing Actinobacteria (Mycolata) in activated sludge foams.Crossref | GoogleScholarGoogle Scholar |

[9]  Thomas, J.A. et al. (2002) Fighting foam with phages? Water Sci. Technol. 46, 511–518.
Fighting foam with phages?Crossref | GoogleScholarGoogle Scholar |

[10]  Liu, M. et al. (2015) Bacteriophages of wastewater foaming-associated filamentous Gordonia reduce host levels in raw activated sludge. Sci. Rep. 5, 13754.
Bacteriophages of wastewater foaming-associated filamentous Gordonia reduce host levels in raw activated sludge.Crossref | GoogleScholarGoogle Scholar |

[11]  Abedon, S.T. et al. (2017) Phage therapy:past, present and future. Front. Microbiol. 8, 981.
Phage therapy:past, present and future.Crossref | GoogleScholarGoogle Scholar |

[12]  Dyson, Z.A. et al. (2015) Lysis to kill: evaluation of the lytic abilities, and genomics of nine bacteriophages infective for Gordonia spp. and their potential use in activated sludge foam biocontrol. PLoS One 10, e0134512.
Lysis to kill: evaluation of the lytic abilities, and genomics of nine bacteriophages infective for Gordonia spp. and their potential use in activated sludge foam biocontrol.Crossref | GoogleScholarGoogle Scholar |

[13]  Petrovski, S. et al. (2011) Characterization of the genome of the polyvalent lytic bacteriophage GTE2, which has potential for biocontrol of Gordonia-, Rhodococcus-, and Nocardia-stabilized foams in activated sludge plants. Appl. Environ. Microbiol. 77, 3923–3929.
Characterization of the genome of the polyvalent lytic bacteriophage GTE2, which has potential for biocontrol of Gordonia-, Rhodococcus-, and Nocardia-stabilized foams in activated sludge plants.Crossref | GoogleScholarGoogle Scholar |

[14]  Petrovski, S. et al. (2012) Small but sufficient: the Rhodococcus phage RRH1 has the smallest known Siphoviridae genome at 14.2 kilobases. J. Virol. 86, 358–363.
Small but sufficient: the Rhodococcus phage RRH1 has the smallest known Siphoviridae genome at 14.2 kilobases.Crossref | GoogleScholarGoogle Scholar |

[15]  Petrovski, S. et al. (2013) Genome sequence and characterization of a Rhodococcus equi phage REQ1. Virus Genes 46, 588–590.
Genome sequence and characterization of a Rhodococcus equi phage REQ1.Crossref | GoogleScholarGoogle Scholar |

[16]  Petrovski, S. et al. (2013) Characterization and whole genome sequences of the Rhodococcus bacteriophages RGL3 and RER2. Arch. Virol. 158, 601–609.
Characterization and whole genome sequences of the Rhodococcus bacteriophages RGL3 and RER2.Crossref | GoogleScholarGoogle Scholar |

[17]  Petrovski, S. et al. (2014) Genome sequence of the Nocardia bacteriophage NBR1. Arch. Virol. 159, 167–173.
Genome sequence of the Nocardia bacteriophage NBR1.Crossref | GoogleScholarGoogle Scholar |

[18]  Dyson, Z.A. et al. (2016) Isolation and characterization of bacteriophage SPI1, which infects the activated-sludge-foaming bacterium Skermania piniformis. Arch. Virol. 161, 149–158.
Isolation and characterization of bacteriophage SPI1, which infects the activated-sludge-foaming bacterium Skermania piniformis.Crossref | GoogleScholarGoogle Scholar |

[19]  Dyson, Z.A. et al. (2015) Three of a Kind: Genetically Similar Tsukamurella Phages TIN2, TIN3, and TIN4. Appl. Environ. Microbiol. 81, 6767–6772.
Three of a Kind: Genetically Similar Tsukamurella Phages TIN2, TIN3, and TIN4.Crossref | GoogleScholarGoogle Scholar |

[20]  Petrovski, S. et al. (2011) Genome sequence and characterization of the Tsukamurella bacteriophage TPA2. Appl. Environ. Microbiol. 77, 1389–1398.
Genome sequence and characterization of the Tsukamurella bacteriophage TPA2.Crossref | GoogleScholarGoogle Scholar |

[21]  Blackall, L.L. and Marshall, K.C. (1989) The mechanism of stabilization of actinomycete foams and the prevention of foaming under laboratory conditions. J. Ind. Microbiol. 4, 181–187.
The mechanism of stabilization of actinomycete foams and the prevention of foaming under laboratory conditions.Crossref | GoogleScholarGoogle Scholar |

[22]  Petrovski, S. et al. (2011) Prevention of Gordonia and Nocardia stabilized foam formation by using bacteriophage GTE7. Appl. Environ. Microbiol. 77, 7864–7867.
Prevention of Gordonia and Nocardia stabilized foam formation by using bacteriophage GTE7.Crossref | GoogleScholarGoogle Scholar |